With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. robotics-automation-compatible. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). When a. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Mode Plate Reader. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. T = I/I 0 and %T = 100 (T). The UV-Plate is compatible with the following plate readers: Bio-Tek PowerWave™ 200 Bio-Tek µQuant™ BioRad UltraMark™ Molecular Devices SPECTRAmax® 190, 250, 340PC, and Plus Ordering Information Cat. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. Compare this item. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. 100 fmol 1536-well (8 µL) 0. the Molecular Devices SPECTRAmax® 190 and 250. Enter the name of the plate type i. The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. minutes and begin reading microplates with seven mouse clicks. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Running both standard spectrophotometer and microplate. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. Learn how you can utilize microplate readers to drive landmark discoveries. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. No. 96-, 384-, and 1536-well microplates. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 100 μL of working reagent was added to each well after the 10-minute preincubation step. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. x. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The CellTiter-Glo assay was used to measure ATP. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. These microplates have a corner notch for use in standard plate readers. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. Dimensions: 18. A. 0, or. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). Microplate format. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. Published Dec 16, 2020 | Updated Jun 22, 2023. Sample by eye within . SpectraDrop High-Throughput Screening (HTS) Kit. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Compare this item. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Loading Buffer was also added to a set of cell-free wells for background controls. EMax. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. 0. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. Molecular Devices' microplate readers. Conventional technique: subjective, time-consuming. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Intuitive graphical user interface makes each assay extremely easy to set. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Features. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. absorbance microplate reader GEA. These robust readers have been placed in labs from Antarctica to the. 1. Shaking: Linear, orbital, and double orbital. x. Please click on the links below to view the Microplate Reader Comparison Tables. The top- and bottom-reading Gemini EM Microplate. 984. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Recertification service. Answer. S. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. the absorbance at 340 nm at 37 °C was measured every 30 s using SpectraMax M5 Microplate. Description: Enter plate name (optional)(2) Wild‑type KRAS inhibits the migration and invasion of pancreatic cancer through the Wnt/β‑catenin pathway Molecular medicine reports January 1, 2023 Hu X, Zhang R, Yao J, Mu B, Zhao C. Please refer to the Obsolete Microplate Reader Models for more information. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. Molecular Devices has provided scientists with . Values are averages from 5 clean plates, each filled with 200 µL water/well. These plasticware can come in a variety of formats, such as skirted, semi-skirted, unskirted, clear, colored, and barcoded. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. 96- or 384-well microplate compatibility. Microplates, cuvettes (via adapter) Detectors. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. ~ Bio-Tek ELx808 Microplate Reader $2,795. 1 OD at 230 nm. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. Cell viability and cytoxicity assays. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. 2 with 1000 cells. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Detection of KASP genotyping reactions. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. * Provides sufficient reagent for 10 plates (96-, 384-well) R8128 FLIPR Membrane Potential Assay Kit Blue (Explorer) (10) vials Component A (blue)* (1) buffer bottle. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. High-throughput, automated dynamic light scattering. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. 0001 ABS (calculated) • Light source: Halogen lamp, 8V/50W • Wavelength range: 405, 450, 492, 630 nm, 4 more filters optional. Wide range of applications The VersaMax reader covers a wide range of applications: Step 1: Turn on your microplate reader. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Part Number. #CA961) Methods. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. x. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. White plates reflect the light, enabling even low levels of Luminescence to be detected. 190. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Get Quote . Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Plate Shaking. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. Unparalleled performance on a personalized platform. #E010) Pyroplate 96-well microplate (ACC cat. Important things to consider when purchasing a multimode microplate reader. . The drawing shows a standard microplate of 15 mm height covered with a lid. Both the P and M injectors were set to deliver 100 µL (96-well plate) or 25 µL (384-well plate), with a 2-second delay and 10-second integration time. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. This reader can also be used as a standard. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. Published Dec 16, 2020 | Updated Dec 16, 2020. Bay Gray powder coated metal back plate, column, and base. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. • 0. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Plate Readers, Fluorometers & Luminometers; Lab Automation; Molecular Diagnostics. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). of 3 wells of a 96-well plate, from 1 of 3 independent experiments. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. VMax . Four wash/rinse bottles are included and configurable for both 96- and 384. They can be read by most microplate readers. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 5A spare fuses, 2 cleaning needles, tubing sets for all bottles, cleaning tool for needles. Pick the best speed, sensitivity, and price for your needs. It is designed to work seamlessly with a variety of 96 well microplate readers, including popular brands such as Agilent™, Berthold™, Biotek™, BMG LABTECH™, Molecular Devices™, Perkin Elmer™, Promega™, Tecan™, and. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. Figure 3. Optimized for fluorescence microplate readers. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. #23225). 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. Supplier: Molecular Devices. Self-righting base, up to 40 degrees past center without dispenser. #R8195. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. 00. Polypropylene deep well plates, round bottom. * Note: Programming the washer with a final aspirate height of 4. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. We would like to show you a description here but the site won’t allow us. 00. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. BMG Labtech - THERMOstar. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Products : Ionworks HT, Ionworks Quattro. When a. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. Silica excitation fiber, glass emission fiber. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. • High antibody-binding surface improves the overall performance in. Uncontrolled Copy for Training and Reference Purposes Only . XTR Rack Pro 96 (Vial Rack Bottom Label 2D Reader) BIO-LAYER INTERFEROMETRY MICROPLATE READING: Forte Bio: Octet HTX: Octet QK384:. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Return to top. 312 gain calibration failed. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. 1 x plates; Instrument dimensions. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. 300 µL of Detergent Compatible Bradford reagent was added to each well. 1. , Corning, NY) or Greiner. the use of a reader calibrated with primary NIST and NMI standards. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. EMax. This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. Anticancer drug assay was also conducted using conventional 96-well plates as controls. • Remove media from each well using a blue pipette tip being careful as not to remove cells. The advanced design of the Sunrise plate reader includes. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. 306 plate air cal. 1. 5. Lucid Scientific. perfect for kinetic readings involving growth curves at 5 seconds per plate. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 5 ng per well. Molecular Devices LLC 96 well plate reader 96 Well Plate Reader, supplied by Molecular Devices LLC, used in various techniques. 1. % transmittance of plates used in a plate reader. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Service parts for these models are not being produced and are no longer in stock. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. Growth medium was then replaced with fatty acid uptake solution. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The variations include adjustable. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Dual injectors for kinetic assays. Create custom fluorescence assays without having to purchase expensive filter sets. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Plate Background OD, which was used for subsequent tests, was measured as follows. 005 OD Normal and Rapid modes, 96- and 384-well plates;. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. Cross-talk <0. Temperature control: 4°C above ambient to 45°C. 00 USD. Quartz 96 Well Plates | 96 Well Plate Flat Bottom | 96 Well Plate Reader | 96 Well Glass. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. #SpectraMax L Config) Methods. The Versamax Microplate Reader is built around a grating monochromator. A = 2 - log 10 (%T). Molecular Devices (UK) Ltd. Component A (2 vials*)Figure 1. Validation made easy -1) Select the correct plate and the wells that you want to measure. 2% in. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. . For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro densitometry of TLC plates and electrophoresis gels. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. as well as all other Molecular Devices' microplate readers. Reader Documentation Version No. with Quartz ABS Plastic. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. the Gemini XPS reader. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). and glow luminescence modes in 96- to 384-well plates. No tools required to assemble. In addition, users can define individual reagents and concentrations to be delivered to each well. 25% and 0. Published Dec 16, 2020 | Updated Dec 16, 2020. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. 0 . 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. #CA961) Methods. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Molecular Devices SpectraMax Plus 384 Absorbance. Highly selective for dsDNA over ssDNA or RNA. The plate reader optics are tunable for. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Absorbance Linearity: 2. 3 OD at 200 nm. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Description. 000 OD ± 1. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. 130009WMicroplate Readers. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 90349). We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. M5. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. 7°C (gradient heating design). Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 4 decades . One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Our. Expand Microplates and select the newly created plate. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. Published Dec 16, 2020 | Updated Dec 16,. This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. ₹ 1,80,000 Get Latest Price. Refurbished. This reader's dual monochromators for. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Time-resolved phosphorescence. Contents of wells were pipetted up and down several times to mix. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. minutes and begin reading microplates with seven mouse clicks. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. BioTek Instruments Synergy Neo2 Multi-Mode Reader Fluorescence Measuring Equipment. The system features 96, 384,. x. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro Software. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. 0 . Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Turn the instrument around so that the back of the instrument is. Back plate and…. Source plate capacity; 1 x plates. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. as well as all other Molecular Devices' microplate readers. Page 1 of 8 . After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Click on the crosshair and move it so that it is centered over the well image. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 00 Add to cart; Molecular Devices EMax Microplate Reader $. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Working in. 96- and 384-well. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. 2b,c. Populate the following info under Description. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. External Dimensions without lid (mm). Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 76358-624. We would like to show you a description here but the site won’t allow us. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMolecular Probes® 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and autofluorescence and so are optimized for fluorescence-based applications. (one set per assay) to clear 96-well microplates. 300 µL of Detergent Compatible Bradford reagent was added to each well. 012 fmol 0. TNF standard curve. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Making it possible to perform kinetic analysis of solid. Optional 16-way manifold. 00.